The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. Available data sets include contigs, scaffolds, chromosomes, ESTs, RNAseq, transcripts and peptides.

This tutorial contains:

  • BLAST basics
    • ✓ E- value new!

  • How to use the tool and interpret its output?
  • Questions and practice exercises
    • ✓ Gene ID lookup new!

For additional documentation visit NCBI BLAST Help section. Topics include user guides, FAQs, videos and much more.

If you want to discuss any issues raised in this tutorial then please contact the help desk.

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